This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pérez, G.
Right arrow Articles by Ramírez, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pérez, G.
Right arrow Articles by Ramírez, L.
Agricola
Right arrow Articles by Pérez, G.
Right arrow Articles by Ramírez, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2009, p. 1427-1436, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01889-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Telomere Organization in the Ligninolytic Basidiomycete Pleurotus ostreatus{triangledown} ,{dagger}

Gúmer Pérez,1 Jasmyn Pangilinan,2 Antonio G. Pisabarro,1 and Lucía Ramírez1*

Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, 31006 Pamplona, Spain,1 U.S. Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California 945982

Received 14 August 2008/ Accepted 21 December 2008

Telomeres are structural and functional chromosome regions that are essential for the cell cycle to proceed normally. They are, however, difficult to map genetically and to identify in genome-wide sequence programs because of their structure and repetitive nature. We studied the telomeric and subtelomeric organization in the basidiomycete Pleurotus ostreatus using a combination of molecular and bioinformatics tools that permitted us to determine 19 out of the 22 telomeres expected in this fungus. The telomeric repeating unit in P. ostreatus is TTAGGG, and the numbers of repetitions of this unit range between 25 and 150. The mapping of the telomere restriction fragments to linkage groups 6 and 7 revealed polymorphisms compatible with those observed by pulsed field gel electrophoresis separation of the corresponding chromosomes. The subtelomeric regions in Pleurotus contain genes similar to those described in other eukaryotic systems. The presence of a cluster of laccase genes in chromosome 6 and a bipartite structure containing a Het-related protein and an alcohol dehydrogenase are especially relevant; this bipartite structure is characteristic of the Pezizomycotina fungi Neurospora crassa and Aspergillus terreus. As far as we know, this is the first report describing the presence of such structures in basidiomycetes and the location of a laccase gene cluster in the subtelomeric region, where, among others, species-specific genes allowing the organism to adapt rapidly to the environment usually map.

* Corresponding author. Mailing address: Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, 31006 Pamplona, Spain. Phone: 34 948169130. Fax: 34 948169732. E-mail: lramirez{at}unavarra.es

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, March 2009, p. 1427-1436, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01889-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»