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Applied and Environmental Microbiology, March 2009, p. 1450-1455, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01944-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection{triangledown}

Bledar Bisha and Byron F. Brehm-Stecher*

Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011

Received 20 August 2008/ Accepted 23 December 2008

A simple adhesive-tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization for rapid culture-independent detection of Salmonella strains. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.

* Corresponding author. Mailing address: 2312 Food Sciences Building, Iowa State University, Ames, IA 50011. Phone: (515) 294-6469. Fax: (515) 294-8181. E-mail: byron{at}iastate.edu

{triangledown} Published ahead of print on 5 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1450-1455, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01944-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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