This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yanai, H.
Right arrow Articles by Ohshima, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yanai, H.
Right arrow Articles by Ohshima, T.
Agricola
Right arrow Articles by Yanai, H.
Right arrow Articles by Ohshima, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2009, p. 1758-1763, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01392-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Sulfolobus tokodaii ST0053 Produces a Novel Thermostable, NAD-Dependent Medium-Chain Alcohol Dehydrogenase{triangledown}

Hisaaki Yanai, Katsumi Doi, and Toshihisa Ohshima*

Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki Higashi-ku, Fukuoka 812-8581, Japan

Received 21 June 2008/ Accepted 4 January 2009

An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed in Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.

* Corresponding author. Mailing address: Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki Higashi-ku, Fukuoka 812-8581, Japan. Phone and fax: 81-92-642-3053. E-mail: ohshima{at}agr.kyushu-u.ac.jp

{triangledown} Published ahead of print on 9 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1758-1763, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01392-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»