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Applied and Environmental Microbiology, March 2009, p. 1778-1781, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.00859-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Tailoring the P450 Monooxygenase Gene for FR-008/Candicidin Biosynthesis{triangledown} ,{dagger}

Shi Chen,2,§,{ddagger} Xiangzhao Mao,1,§,¶ Yaling Shen,1 Yongjun Zhou,2 Jialiang Li,2 Lianrong Wang,2 Xinyi Tao,1 Liang Yang,1 Yuxiao Wang,1 Xiufen Zhou,2 Zixin Deng,2* and Dongzhi Wei1*

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China,1 Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China2

Received 15 April 2008/ Accepted 6 January 2009

Tailoring steps are often important for the activity of mature antibiotics. Here, we report that novel decarboxylated FR-008/candicidin derivatives were obtained from the P450 monooxygenase gene fscP mutant of Streptomyces sp. strain FR-008. The toxicity of decarboxylated FR-008/candicidin derivatives has been shown to be greatly reduced compared to that of wild-type FR-008/candicidin.

* Corresponding author. Mailing address for Zixin Deng: Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China. Phone: 86-21-62933404. Fax: 86-21-62932418. E-mail: zxdeng{at}sjtu.edu.cn. Mailing address for Dongzhi Wei: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China. Phone: 86-21-64252981. Fax: 86-21-64250068. E-mail: dzhwei{at}ecust.edu.cn

{triangledown} Published ahead of print on 9 January 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

§ S.C. and X.M. are co-first authors and contributed equally to this work.

{ddagger} Present address: Department of Cell Biology, Harvard Medical School, Boston, MA 02115.

Present address: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China.

Applied and Environmental Microbiology, March 2009, p. 1778-1781, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.00859-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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