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Applied and Environmental Microbiology, March 2009, p. 1782-1785, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01887-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Transcriptional Regulation and Signal-Peptide-Dependent Secretion of Exolevanase (LsdB) in the Endophyte Gluconacetobacter diazotrophicus{triangledown}

Carmen Menéndez,1 Alexander Banguela,1 Jesús Caballero-Mellado,2 and Lázaro Hernández1*

Laboratorio Interacciones Planta-Microorganismos, Centro de Ingeniería Genética y Biotecnología (CIGB), Apartado 6162, Habana 10600, Cuba,1 Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México (UNAM), Apartado Postal 565-A, Cuernavaca, Morelos, México2

Received 13 August 2008/ Accepted 6 January 2009

Gluconacetobacter diazotrophicus utilizes plant sucrose with a constitutively expressed levansucrase (LsdA), producing extracellular levan, which may be degraded under energetically unfavored conditions. Reverse transcriptase-PCR analysis revealed that lsdA and the downstream exolevanase gene (lsdB) form an operon. lsdB transcription was induced during growth with low fructose concentrations (0.44 to 33 mM) and repressed by glucose. Transport of LsdB to the periplasm involved N-terminal signal peptide cleavage. Type II secretion mutants failed to transfer LsdB across the outer membrane, impeding levan hydrolysis.

* Corresponding author. Mailing address: Laboratorio Interacciones Planta-Microorganismos, Centro de Ingeniería Genética y Biotecnología (CIGB), Apartado 6162, Habana 10600, Cuba. Phone: 53-7-2716022. Fax: 53-7-331779. E-mail: lazaro.hernandez{at}cigb.edu.cu

{triangledown} Published ahead of print on 9 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1782-1785, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01887-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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