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Applied and Environmental Microbiology, April 2009, p. 2446-2452, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02310-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Filamentous Bacterium Eikelboom Type 0092 in Activated Sludge Plants in Australia Is a Member of the Phylum Chloroflexi{triangledown}

Lachlan Speirs, Tadashi Nittami, Simon McIlroy, Sarah Schroeder, and Robert J. Seviour*

Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3552, Australia

Received 8 October 2008/ Accepted 3 February 2009

Molecular data show that the filamentous bacterium Eikelboom type 0092, frequently seen in Australian activated sludge plants, is a member of the phylum Chloroflexi. Fluorescence in situ hybridization (FISH) probes designed against cloned 16S rRNA sequences from a full-scale enhanced biological phosphate removal-activated sludge plant community, where this was a dominant filament morphotype, suggest that it can exist as two variants, differing in their trichome diameter. When applied to samples from several treatment plants in eastern Australia, each FISH probe targeted only the type 0092 filament morphotype against which it was designed. The patterns of FISH signals generated with both were consistent with the ribosomes not being evenly distributed but arranged as intracellular aggregates. The FISH survey data showed that these two variants appeared together in most but not all of the plants examined. None stained positively for intracellular presence of either poly-β-hydroxyalkanoates or polyphosphate.

* Corresponding author. Mailing address: Biotechnology Research Centre, La Trobe University, P.O. Box 199, Bendigo, Victoria 3552, Australia. Phone: 61 3 5444 7456. Fax: 61 3 5444 7476. E-mail: r.seviour{at}latrobe.edu.au

{triangledown} Published ahead of print on 13 February 2009.

Applied and Environmental Microbiology, April 2009, p. 2446-2452, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02310-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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