This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meijnen, J.-P.
Right arrow Articles by Ruijssenaars, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meijnen, J.-P.
Right arrow Articles by Ruijssenaars, H. J.
Agricola
Right arrow Articles by Meijnen, J.-P.
Right arrow Articles by Ruijssenaars, H. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2009, p. 2784-2791, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02713-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Establishment of Oxidative D-Xylose Metabolism in Pseudomonas putida S12{triangledown}

Jean-Paul Meijnen,1,2,3,4* Johannes H. de Winde,1,3,4 and Harald J. Ruijssenaars2,3,4

Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands,1 TNO-Quality of Life, Business Unit Food and Biotechnology Innovations, Julianalaan 67, 2628 BC Delft, The Netherlands,2 B-Basic, Julianalaan 67, 2628 BC Delft, The Netherlands,3 Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 5057, 2600 GA Delft, The Netherlands4

Received 26 November 2008/ Accepted 28 February 2009

The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose–1) and a maximum growth rate of 0.21 h–1. Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on D-xylose. Only the xylD gene, encoding D-xylonate dehydratase, proved to be essential for establishing an oxidative D-xylose catabolic pathway in P. putida S12. The growth performance on D-xylose was, however, greatly improved by coexpression of xylXA, encoding 2-keto-3-deoxy-D-xylonate dehydratase and {alpha}-ketoglutaric semialdehyde dehydrogenase, respectively. The endogenous periplasmic glucose dehydrogenase (Gcd) of P. putida S12 was found to play a key role in efficient oxidative D-xylose utilization. Gcd activity not only contributes to D-xylose oxidation but also prevents the intracellular accumulation of toxic catabolic intermediates which delays or even eliminates growth on D-xylose.

* Corresponding author. Mailing address: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands. Phone: (31) 15-2789871. Fax: (31) 15-2782355. E-mail: J.P.Meijnen{at}tudelft.nl

{triangledown} Published ahead of print on 6 March 2009.

Applied and Environmental Microbiology, May 2009, p. 2784-2791, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02713-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»