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Applied and Environmental Microbiology, May 2009, p. 2931-2935, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02108-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Antibody Recognition Force Microscopy Shows that Outer Membrane Cytochromes OmcA and MtrC Are Expressed on the Exterior Surface of Shewanella oneidensis MR-1{triangledown}

Brian H. Lower,1* Ruchirej Yongsunthon,2 Liang Shi,3 Linda Wildling,4 Hermann J. Gruber,4 Nicholas S. Wigginton,5 Catherine L. Reardon,3 Grigoriy E. Pinchuk,3 Timothy C. Droubay,3 Jean-François Boily,6 and Steven K. Lower1

The Ohio State University, Columbus, Ohio 43210,1 Corning Incorporated, Corning, New York 14831,2 Pacific Northwest National Laboratory, Richland, Washington 99352,3 Johannes Kepler University of Linz, A-4040 Linz, Austria,4 Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland,5 Umeå University, SE-901 87 Umeå, Sweden6

Received 11 September 2008/ Accepted 27 February 2009

Antibody recognition force microscopy showed that OmcA and MtrC are expressed on the exterior surface of living Shewanella oneidensis MR-1 cells when Fe(III), including solid-phase hematite (Fe2O3), was the terminal electron acceptor. OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance.

* Corresponding author. Mailing address: School of Environment and Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd., Columbus, OH 43210. Phone: (614) 247-1676. Fax: (614) 292-7432. E-mail: Lower.30{at}osu.edu

{triangledown} Published ahead of print on 13 March 2009.

Applied and Environmental Microbiology, May 2009, p. 2931-2935, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02108-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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