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Applied and Environmental Microbiology, May 2009, p. 2958-2963, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02325-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cloning and Characterization of the Biosynthetic Gene Cluster for Tomaymycin, an SJG-136 Monomeric Analog{triangledown} ,{dagger}

Wei Li, ShenChieh Chou, Ankush Khullar, and Barbara Gerratana*

Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742

Received 9 October 2008/ Accepted 25 February 2009

Tomaymycin produced by Streptomyces achromogenes is a naturally produced pyrrolobenzodiazepine (PBD). The biosynthetic gene cluster for tomaymycin was identified and sequenced. The gene cluster analysis reveals a novel biosynthetic pathway for the anthranilate moiety of PBDs. Gene replacement and chemical complementation studies were used to confirm the proposed biosynthetic pathway.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, Bldg. 091, University of Maryland, College Park, MD 20742. Phone: (301) 405-1541. Fax: (301) 314-9121. E-mail: bgerrata{at}umd.edu

{triangledown} Published ahead of print on 6 March 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2009, p. 2958-2963, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02325-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, W., Khullar, A., Chou, S., Sacramo, A., Gerratana, B. (2009). Biosynthesis of Sibiromycin, a Potent Antitumor Antibiotic. Appl. Environ. Microbiol. 75: 2869-2878 [Abstract] [Full Text]  


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