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Applied and Environmental Microbiology, May 2009, p. 2991-2995, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.00181-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Glycerol Utilization Gene Cluster in Streptomyces clavuligerus{triangledown} ,{dagger}

Sonia Baños,1 Rosario Pérez-Redondo,1,2 Bert Koekman,3 and Paloma Liras1,2*

Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain,1 Instituto de Biotecnología, INBIOTEC, Parque Científico de León, 24006 León, Spain,2 DSM Anti-Infectives B.V., Delft, The Netherlands3

Received 26 January 2009/ Accepted 4 March 2009

The Streptomyces clavuligerus ATCC 27064 glycerol cluster gylR-glpF1K1D1 is induced by glycerol but is not affected by glucose. S. clavuligerus growth and clavulanic acid production are stimulated by glycerol, but this does not occur in a glpK1-deleted mutant. Amplification of glpK1D1 results in transformants yielding larger amounts of clavulanic acid in the wild-type strain and in overproducer S. clavuligerus Gap15-7-30 or S. clavuligerus {Delta}relA strains.

* Corresponding author. Mailing address: Área de Microbiología, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain. Phone: (34) 987 291504. Fax: (34) 987 291506. E-mail: paloma.liras{at}unileon.es

{triangledown} Published ahead of print on 13 March 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2009, p. 2991-2995, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.00181-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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