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Appl. Environ. Microbiol. doi:10.1128/AEM.00019-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Characterization of Staphylococcus aureus isolated from buffalo, bovine, ovine, and caprine milk samples from Rio de Janeiro State, Brazil

Laboratório de Genética Molecular, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa (ITQB/UNL), Oeiras, Portugal; Laboratório de Sanidade Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense-UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil; Laboratory of Microbiology, The Rockefeller University, New York, NY, USA

^* To whom correspondence should be addressed. Email: lencash{at}mail.rockefeller.edu.

	Abstract

Eighty-four staphylococcal isolates were obtained from milk samples of cows, sheep, goats, and buffalo with subclinical mastitis and from colonization samples of ostriches. The animals were hosted in 18 small dairy herds and an ostrich breeding located in 10 municipalities of the State of Rio de Janeiro, Brazil. Thirty isolates were identified as S. aureus by biochemical and molecular techniques and were comparatively characterized by phenotypic and genotypic methods. The molecular characterization by pulsed-field gel electrophoresis (PFGE), spa typing and multilocus sequence typing (MLST) revealed five clonal types (PFGE A - spa type t359 - ST747, PFGE B - spa type t1180 - ST750, PFGE C - spa type t605 - ST126, PFGE D - spa type t127 - ST751, and PFGE F - spa type t002 - ST5). None of the isolates harbored the Panton-Valentine leukocidin or exfoliative toxin D genes. The detection of a major clone A (63%) in different herds, among all different animal species, and in infection and colonization samples evidenced its geographical spread among the Rio de Janeiro State and no host preference among the animal species. Comparison with S. aureus from human origin suggested that all but one clone found in the present study might be animal specific.

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