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Appl. Environ. Microbiol. doi:10.1128/AEM.00075-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Functional nodFE genes are present in Sinorhizobium sp. strain MUS10, a symbiont of tropical legume Sesbania rostrata

Hari B. Krishnan* and Demosthenis Chronis

Plant Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service and Department of Plant Sciences, University of Missouri, Columbia, MO 65211

* To whom correspondence should be addressed. Email: KrishnanH{at}missouri.edu.


   Abstract

We have cloned the nodFE operon from Sinorhizobium sp. strain MUS10. MUS10 NodF shows sequence homology to acyl-carrier protein and enables S. meliloti nodF mutant to effectively nodulate alfalfa. Our results demonstrate the occurrence of nodFE in a symbiont that nodulates a legume host not belonging to Galegoid group.







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