AEM Accepts, published online ahead of print on 3 August 2007

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Appl. Environ. Microbiol. doi:10.1128/AEM.00325-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Use of Disposable Water Filter for Prevention of False Positive Result Due to Non-Tuberculosis Mycobacteria (NTM) in a Clinical Laboratory Performing Routine Acid-Fast Stain for Tuberculosis

Hui-Zin Tu, Chiao-Shan Chen, Tsi-Shu Huang, Wen-Kuei Huang, Yao-shen Chen, Yung-ching Liu, and Yusen Eason Lin^*

Kaohsiung Veterans General Hospital, Kaohsiung, TAIWAN; National Kaohsiung Normal University, Kaohsiung, TAIWAN

^* To whom correspondence should be addressed. Email: easonlin{at}nknucc.nknu.edu.tw.

A point-of-use 0.2µm filter was evaluated for elimination of NTM in laboratory water to reduce the false positive AFB stain. Use of point-of-use filter can significantly reduce the false positive rate to 1.2% compared to samples treated by tap water (10.7%) and deionized water (8.7%).