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Appl. Environ. Microbiol. doi:10.1128/AEM.00419-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Pathotyping Escherichia coli using miniaturised DNA-microarrays

Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, U.K.; CLONDIAG Chip Technologies GmbH, Jena, Germany; Institute of Veterinary Bacteriology, Vetsuisse Faculty of the University of Bern, Switzerland

^* To whom correspondence should be addressed. Email: m.anjum{at}vla.defra.gsi.gov.uk.

	Abstract

Detection of virulence determinants harboured by pathogenic E. coli is important in establishing the pathotype responsible for infection. A sensitive and specific miniaturised virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in future for high throughput use.

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