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AEM Accepts, published online ahead of print on 17 August 2007
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Appl. Environ. Microbiol. doi:10.1128/AEM.00715-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification of genes that confer sediment fitness to Desulfovibrio desulfuricans G20

Qingwei Luo, Jennifer L. Groh, Jimmy D. Ballard, and Lee R. Krumholz*

Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019 and Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190

* To whom correspondence should be addressed. Email: krumholz{at}ou.edu.


   Abstract

Signature-tagged-mutants of Desulfovibrio desulfuricans G20 were screened and 97 genes crucial for sediment fitness were identified. These genes belong to functional categories including signal transduction, binding and transport, insertion elements and others. Mutants in genes encoding proteins involved in amino acid biosynthesis, hydrogenase activity and DNA repair were further characterized.







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