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Appl. Environ. Microbiol. doi:10.1128/AEM.01037-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Characterization and Synergistic Interactions of Fibrobacter succinogenes Glycoside Hydrolases

Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada

^* To whom correspondence should be addressed. Email: cforsber{at}uoguelph.ca.

	Abstract

The objective of this study was to characterize F. succinogenes glycoside hydrolases from different glycoside hydrolase families and to study their synergistic interactions. The gene encoding a major endoglucanase (endoglucanase 1) of F. succinogenes S85 was identified as cel9B from the genome sequence by reference to internal amino acid sequences of the purified native enzyme. Cel9B and two other glucanases from different families, Cel5H and Cel8B were cloned, overexpressed, and the proteins purified and characterized. These proteins in conjunction with two predominant cellulases, Cel10A-a chloride-stimulated cellobiosidase, and Cel51A formerly known as endoglucanase 2 (or CelF), were assayed in various combinations to assess their synergistic interactions using ball-milled cellulose. The degree of synergism ranged from 0.6 to 3.7. The two predominant endoglucanases produced by F. succinogenes, Cel9B and Cel51A, were shown to have a synergistic effect of up to 1.67. Cel10A showed little synergy in combination with Cel9B and Cel51A. Mixtures containing all the enzymes gave a higher synergism degree than those containing 2 or 3 enzymes, which reflected the complementarity in their modes of action as well as substrate specificities.

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