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Appl. Environ. Microbiol. doi:10.1128/AEM.01157-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases

Mi-Hwa Lee, Choong-Hwan Lee, Tae-Kwang Oh, Jae Kwang Song*, and Jung-Hoon Yoon*

Korea Research Institute of Bioscience and Biotechnology, and Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea

* To whom correspondence should be addressed. Email: ajee{at}krict.re.kr, jhyoon{at}kribb.re.kr.


   Abstract

We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.







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