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Appl. Environ. Microbiol. doi:10.1128/AEM.01362-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Engineering Deinococcus radiodurans R1 for bioprecipitation of uranium from dilute nuclear waste

Deepti Appukuttan, Amara Sambasiva Rao, and Shree Kumar Apte*

Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, India

* To whom correspondence should be addressed. Email: sksmbd{at}barc.gov.in.


  Abstract

Genetic engineering of radiation resistant organisms to recover radionuclides/heavy metals from radioactive wastes is an attractive proposition. We have constructed a Deinococcus radiodurans strain harboring phoN, a gene encoding a non-specific acid phosphatase, obtained from a local isolate of Salmonella typhi. The recombinant strain expressed a ~27 kDa active PhoN protein and efficiently precipitated over 90% of uranium in 6 h from a 0.8 mM uranyl nitrate solution. The engineered strain retained uranium bioprecipitation ability even after exposure to 6 kGy of 60Co-gamma rays. The PhoN expressing D. radiodurans offers an effective and eco-friendly in situ approach to biorecovery of uranium from dilute nuclear waste.




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