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Appl. Environ. Microbiol. doi:10.1128/AEM.01496-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification and characterization of a fructose PTS in Bifidobacterium breve UCC2003

Department of Microbiology and Alimentary Pharmabiotic Centre, University of Ireland, Cork, Western Road, Cork, Ireland; Laboratoire de Microbiologie et Génétique Moléculaire, INRA-CNRS-INA PG, CBAI, route de Thiverval, 78850 Thiverval-Grignon, France

^* To whom correspondence should be addressed. Email: d.vansinderen{at}ucc.ie.

	Abstract

In silico analysis of the Bifidobacterium breve UCC2003 genome allowed the identification of four genetic loci, each of which specifying a putative EII protein of a PTS (phosphoenolpyruvate:sugar phosphotransferase system). The EII encoded by fruA, a clear homologue of the unique EIIBCA enzyme specified by the Bifidobacterium longum NCC2705 genome, was studied in more detail. The fruA gene is part of an operon, which contains fruT, predicted to encode a homologue of the Bacillus subtilis antiterminator LicT. Transcriptional analysis showed that the fru operon is induced by fructose. The genetic structure, complementation studies and the observed transcription pattern of the fru operon suggests that the encoded EII in B. breve is involved in fructose transport and that its expression is controlled by an antiterminator mechanism. Biochemical studies unequivocally demonstrated that FruA phosphorylates fructose at the C6-position.

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