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Appl. Environ. Microbiol. doi:10.1128/AEM.01715-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Phylogenetic Analysis of Bacteria Preserved in a Permafrost Ice Wedge for 25,000 Years

Laboratory of Applied Microbiology, Graduate School of Agriculture, Institute of Low Temperature Science, Hokkaido University, and Hokkaido Study Center, University of the Air, Kita-ku, Hokkaido, Graduate School of Engineering, Center for Chronological Research, Nagoya University, Chikusa-ku, Aichi-ken, Japan; Cold Regions Research and Engineering Laboratory, Fort Wainwright, Alaska, USA

^* To whom correspondence should be addressed. Email: asanok{at}chem.agr.hokudai.ac.jp.

	Abstract

Phylogenetic analysis of bacteria preserved within an ice wedge from the Fox Permafrost Tunnel was undertaken by cultivation and molecular techniques. The radiocarbon age of the ice wedge was determined. Our results suggest that the bacteria in the ice wedge adapted to the frozen conditions have survived for 25,000 years.