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Appl. Environ. Microbiol. doi:10.1128/AEM.01831-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Endomicrobia and other bacteria associated with the hindgut of Dermolepida albohirtum larvae

School of Integrative Biology, The University of Queensland, St. Lucia, Qld 4072, Australia; BSES Limited, PO Box 86, Indooroopilly, Qld 4068, Australia; Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Qld 4072, Australia

^* To whom correspondence should be addressed. Email: scott.oneill{at}uq.edu.au.

	Abstract

Symbiotic bacteria residing in the hindgut chamber of scarab beetle larvae may be useful in paratransgenic approaches to reduce larval root-feeding activities in agricultural crops. We compared the bacterial community profiles associated with the hindgut wall of individual Dermolepida albohirtum third-instar larvae over two years and with their plant-root food source among different geographic regions. Denaturing gradient gel electrophoresis (DGGE) analysis was used with universal and Actinobacteria specific 16S rRNA primers to reveal a number of taxa that were found consistently in all D. albohirtum larvae but not in their food source, sugarcane roots. These taxa included representatives from the Endomicrobia, Firmicutes, Proteobacteria and Actinobacteria, and are related to bacteria previously described from the intestines of other scarab larvae and termites. These universally distributed taxa have the potential to form vertically transmitted symbiotic associations with these insects.

This article has been cited by other articles:

• Pittman, G. W., Brumbley, S. M., Allsopp, P. G., O'Neill, S. L. (2008). Assessment of Gut Bacteria for a Paratransgenic Approach To Control Dermolepida albohirtum Larvae. Appl. Environ. Microbiol. 74: 4036-4043 [Abstract] [Full Text]