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Appl. Environ. Microbiol. doi:10.1128/AEM.01992-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Density Gradient Enrichment of Escherichia coli Conditional msbA Mutants

Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803

^* To whom correspondence should be addressed. Email: wdoerr{at}lsu.edu.

	Abstract

Insight into the mechanism of lipid transport to the outer membrane of Gram-negative bacteria has been hampered by the lack of an effective genetic screen for defective mutants. This work demonstrates an enrichment of conditional mutants defective in lipopolysaccharide export by Ludox density gradient centrifugation and selection for detergent resistance. New temperature sensitive mutants with lipid export defects were isolated with single missense mutations in msbA. These results demonstrate the power of this approach to study lipid export in E. coli.

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