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Appl. Environ. Microbiol. doi:10.1128/AEM.02208-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Temporal Transcriptomic Microarray Analysis of Dehalococcoides ethenogenes Strain 195 During the Transition into the Stationary Phase

Department of Civil and Environmental Engineering, University of California, Berkeley CA 94720-1710; Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720; School of Public Health, University of California, Berkeley, CA 94720; Department of Microbiology, Cornell University, Ithaca, NY 14853

^* To whom correspondence should be addressed. Email: alvarez{at}ce.berkeley.edu.

	Abstract

Dehalococcoides bacteria can reductively dehalogenate a wide range of halogenated organic pollutants. In this study, DNA microarrays were applied to monitor dynamic changes in the transcriptome as Dehalococcoides ethenogenes strain 195 transitioned from exponential growth into the stationary phase. In total, 415 non-redundant genes were identified as differentially expressed. As expected, genes involved with translation and energy metabolism were down-regulated while genes involved with general stress response, transcription, and signal transduction were up-regulated. Unexpected, however, was the 8- to 10-fold up-regulation of four putative reductive dehalogenases (RDases) (DET0173, DET0180, DET1535, and DET1545). Another unexpected finding was the up-regulation of a large number of genes located within integrated elements, including a putative prophage and a muti-copy transposon. Finally, genes encoding the dominant hydrogenase-RDase respiratory chain of this strain (Hup and TceA) were expressed at stable levels throughout the experiment, providing molecular evidence that strain 195 can uncouple dechlorination from net growth.

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