This Article Full Text (PDF) Other Versions of this Article: AEM.02233-06v1 73/10/3183    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ishida, T. Articles by Kato, J. Search for Related Content PubMed PubMed Citation Articles by Ishida, T. Articles by Kato, J. Agricola Articles by Ishida, T. Articles by Kato, J.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol. doi:10.1128/AEM.02233-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Inhibition of Quorum Sensing in Pseudomonas aeruginosa by N-Acyl Cyclopentylamides

Department of Molecular Biotechnology, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Department of Applied Chemistry, Utsunomiya University, Utsunomiya, Tochigi 321-8585, Department of Biotechnology, Osaka University, Suita, Osaka 565-0871, Japan

^* To whom correspondence should be addressed. Email: jun{at}hiroshima-u.ac.jp.

	Abstract

N-Octanoyl cyclopentylamide (C8-CPA) was found to moderately inhibit quorum sensing in Pseudomonas aeruginosa PAO1. To obtain more powerful inhibitors, a series of structural analogs of C8-CPA were synthesized and examined for their ability to inhibit quorum sensing in P. aeruginosa PAO1. The lasB-lacZ and rhlA-lacZ reporter assays revealed that the chain length and the ring structure were critical for C8-CPA analogs to inhibit quorum sensing. N-Decanoyl cyclopentylamide (C10-CPA) was found to be the strongest inhibitor and its concentrations required for half-maximal inhibition for lasB-lacZ and rhlA-lacZ expression were 80 and 90 µM, respectively. C10-CPA also inhibited production of virulence factors including elastase, pyocyanin, and rhamnolipid, and biofilm formation without affecting growth of P. aeruginosa PAO1. C10-CPA inhibited induction of both lasI-lacZ by N-(3-oxododecanoyl)-L-homoserine lactone (PAI1) and rhlA-lacZ by N-butanoyl-L-homoserine lactone (PAI2) in the lasI rhlI mutant of P. aeruginosa PAO1, indicating that C10-CPA interferes with the las and rhl quorum sensing systems via inhibiting interaction between their response regulators (LasR and RhlR) and autoinducers.

This article has been cited by other articles:

• Morohoshi, T., Shiono, T., Takidouchi, K., Kato, M., Kato, N., Kato, J., Ikeda, T. (2007). Inhibition of Quorum Sensing in Serratia marcescens AS-1 by Synthetic Analogs of N-Acylhomoserine Lactone. Appl. Environ. Microbiol. 73: 6339-6344 [Abstract] [Full Text]