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Appl. Environ. Microbiol. doi:10.1128/AEM.02538-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Bioaccumulation of palladium by Desulfovibrio fructosovorans and hydrogenase deficient mutants

School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, England, United Kingdom; Bioenérgétique et Ingénierie des Protéines, CNRS, 31 Chemin Cedex 20, Joseph Aiguier, 13402 Marseille France

^* To whom correspondence should be addressed. Email: L.E.Macaskie{at}bham.ac.uk.

	Abstract

Wild type Desulfovibrio fructosovorans and three hydrogenase-negative mutants reduced Pd(II) to Pd(0). The location of Pd(0)-nanoparticles on the cytoplasmic membrane of the mutant retaining only cytoplasmic membrane-bound hydrogenase is strong evidence for the role of hydrogenases in Pd(0)-deposition. Hydrogenase activity was retained at acidic pH, shown previously to favour Pd(0) deposition.