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Appl. Environ. Microbiol. doi:10.1128/AEM.02561-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Development of a semi-quantitative degenerate real-time PCR-based assay for the estimation of butyryl-CoA CoA-transferase genes in complex bacterial samples

Rowett Research Institute, Gut Health Division, Microbial Ecology Group, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom

^* To whom correspondence should be addressed. Email: p.louis{at}rowett.ac.uk.

	Abstract

We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-CoA CoA-transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon and thus this assay estimates the butyrate-producing capacity of the microbiota.