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Appl. Environ. Microbiol. doi:10.1128/AEM.02603-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Simultaneous detection of Pseudomonas fragi, P. lundensis and P. putida from meat by a multiplex PCR assay targeting the carA gene

Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, via Università 100, 80055 Portici (NA) Italy

^* To whom correspondence should be addressed. Email: ercolini{at}unina.it.

	Abstract

Species-specific primers and a multiplex PCR assay were developed for the simultaneous identification and differentiation of P. fragi, P. lundensis and P. putida based on the co-amplification of different portions of the small subunit of the carbamoyl phosphate synthase gene (carA). The carA multiplex PCR was used to detect the presence of the three Pseudomonas species from beef, chicken and pork samples and proved to be effective in showing their evolution during the storage of meat.