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Appl. Environ. Microbiol. doi:10.1128/AEM.02623-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Comparison of Transcription-Mediated Amplification and growth-based methods for the quantitation of Enterococcus in environmental waters

MLT Research Ltd., 5 Chiltern Close, Cardiff CF14 5DL, UK (A subsidiary of Gen-Probe Incorporated); University of North Carolina at Chapel Hill, Institute of Marine Sciences, Morehead City, NC 28557, USA; Gen-Probe Incorporated, 10210 Genetic Center Drive, San Diego, CA 92121, USA

	Abstract

An assay based on transcription-mediated amplification (TMA) technology was used to quantitate fecal indicator bacteria, Enterococcus, in environmental water samples. The results generated by this and two growth-based methods relative to the 104 MPN or CFU per 100 ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 hours from sample collection to result) and quantitation of Enterococcus when compared to the growth-based methods.