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Appl. Environ. Microbiol. doi:10.1128/AEM.02630-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Recovery, purification and cloning of high molecular weight DNA from soil microorganisms

	Abstract

We describe here an improved method for isolating, purifying and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that co-purified with the metagenomic DNA were removed by incubating plugs with a high salt and formamide solution. This method was used to construct large-insert soil metagenomic libraries.