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Appl. Environ. Microbiol. doi:10.1128/AEM.02660-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Determination of glycosyl transferase specificities for the E. coli O111 O antigen using a generic approach

School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia

^* To whom correspondence should be addressed. Email: reeves{at}mmb.usyd.edu.au.

	Abstract

We describe a strain developed to facilitate determination of GT specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the E. coli O111 O antigen glycosyl transferase genes.