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Appl. Environ. Microbiol. doi:10.1128/AEM.02702-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

High-Resolution DNA Melt Curve Analysis of the Clustered Regularly Interspaced Short Palindromic Repeat Locus of Campylobacter jejuni

Erin P. Price, Helen Smith, Flavia Huygens, and Philip M. Giffard*

Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.; Queensland Health Scientific Services, Coopers Plains, Australia

* To whom correspondence should be addressed. Email: p.giffard{at}qut.edu.au.


  Abstract

A novel method for genotyping the clustered regularly interspaced short palindromic repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods, and emphasizes the potential of HRM for genotyping short sequence repeats in other species.




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