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Appl. Environ. Microbiol. doi:10.1128/AEM.02704-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Co-metabolic Degradation of Dibenzofuran and Dibenzothiophene by a Newly Isolated Carbazole-degrading Sphingomonas sp. Strain

Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240; Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080; State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China

^* To whom correspondence should be addressed. Email: pingxu{at}sdu.edu.cn.

	Abstract

A carbazole-utilizing bacterium was isolated by enrichment from a petroleum contaminated soil. The isolate, designated as Sphingomonas sp. XLDN2-5, could utilize carbazole (CA) as the sole source of carbon, nitrogen and energy. Washed cells of XLDN2-5 were shown to be capable of degrading dibenzofuran (DBF) and dibenzothiophene (DBT). Examination of metabolites suggested that XLDN2-5 degraded DBF to 2-hydroxy-6-(2-hydroxyphenyl)-6-oxo-2,4-hexadienic acid and subsequently to salicylic acid through the angular dioxygenation pathway. In contrast to DBF, strain XLDN2-5 could transform DBT through the ring cleavage and sulfoxidation pathways. Sphingomonas sp. XLDN2-5 could co-metabolically degrade DBF and DBT in the growing system using CA as a substrate. After 40 h incubation, 90% of DBT was transformed and CA and DBF were completely removed. These results suggested that strain XLDN2-5 might be useful in the bioremediation of environments contaminated by these compounds.

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