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Appl. Environ. Microbiol. doi:10.1128/AEM.02730-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Comparative transcriptome analysis of Listeria monocytogenes strains of the two major lineages reveals differences in virulence, cell wall and stress response

Unité de Génomique des Microorganismes Pathogénes and CNRS URA 2171, Unité des Interactions Bactéries-Cellules, Institut Pasteur, 25 - 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, INSERM U604, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, INRA USC2020, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France, Equipe Qualité et Sécurité des Aliments (QuaSA), INRA de Clermont-Ferrand/Theix, F-63122 Saint-Genés Champanelle, Computational Biology Group, Institute for Systems Biology, 1441 North 34th Street, Seattle, USA, UR484 Microbiologie, Departamento de Microbiologia, Instituto de Ciências Biomédicas da Universidade de São Paulo, 05508-900, Sãao Paulo, Brazil, Laboratoire de Microbiologie, INRA UMR1232, 1 Esplanade Erasme, F-21000 Dijon, France

^* To whom correspondence should be addressed. Email: cbuch{at}pasteur.fr.

	Abstract

Listeria monocytogenes is a food-borne, opportunistic bacterial pathogen, causing a wide spectrum of diseases in humans and animals like meningitis, septicemia, abortion or gastroenteritis. Among the 13 L. monocytogenes serovars (sv) described, human listeriosis is mostly associated with strains of sv 4b, 1/2b, and 1/2a. Within the species L. monocytogenes three phylogenetic lineages are described. Sv 1/2a belongs to phylogenetic lineage I, while sv 4b and 1/2b group in phylogenetic lineage II. To explore the role of gene expression in the adaptation of L. monocytogenes strains of these two major lineages to different environments as well as in virulence, we performed whole-genome expression profiling of six L. monocytogenes isolates of sv 4b, 1/2b and 1/2a of distinct origins, using a newly constructed Listeria multi-genome DNA array. Comparison of the global gene expression profiles revealed differences among strains. The expression profiles of two strains having distinct LD50, as assessed in the mouse model, were further analyzed. Gene Ontology (GO) term enrichment analysis of the differentially expressed genes identified differences in protein, nucleic acid, carbon metabolism and virulence-related gene expression. Comparison of the expression profiles of the core genome of all strains revealed differences of the two lineages with respect to cell wall synthesis, the stress related sigma B regulon and virulence related genes. These findings suggest different patterns of interaction with host cells and the environment, key factors for host colonization and survival in the environment.

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