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Appl. Environ. Microbiol. doi:10.1128/AEM.02765-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Bacterial degradation of DEET: cloning and heterologous expression of DEET hydrolase

Department of Microbiology and Institute for Comparative and Environmental Toxicology, Cornell University, Ithaca, NY 14853

^* To whom correspondence should be addressed. Email: agh5{at}cornell.edu.

	Abstract

Pseudomonas putida DTB grew aerobically with DEET (N,N-diethyl-m-toluamide) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and meta ring cleavage. A gene from DTB, dthA, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.