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AEM Accepts, published online ahead of print on 16 February 2007
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Appl. Environ. Microbiol. doi:10.1128/AEM.02844-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A mariner-Based Transposition System for Listeria monocytogenes

Min Cao, Alan Pavinski Bitar, and Hélène Marquis*

Department of Microbiology and Immunology, Cornell University, Ithaca, NY, 14853

* To whom correspondence should be addressed. Email: hm72{at}cornell.edu.


   Abstract

In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition, a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high throughput transposon mutagenesis in L. monocytogenes.




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