AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] --
AEM Accepts, published online ahead of print on 4 April 2008
This Article
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.02866-07v1
74/11/3387    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stoitsova, S. O.
Right arrow Articles by Weingart, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stoitsova, S. O.
Right arrow Articles by Weingart, H.
Agricola
Right arrow Articles by Stoitsova, S. O.
Right arrow Articles by Weingart, H.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol. doi:10.1128/AEM.02866-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Characterization of the RND-type multidrug efflux pump MexAB-OprM from the plant pathogen Pseudomonas syringae

Savina O. Stoitsova, Yvonne Braun, Matthias S. Ullrich, and Helge Weingart*

Jacobs University Bremen, School of Engineering and Science, Bremen, Germany

* To whom correspondence should be addressed. Email: h.weingart{at}jacobs-university.de.


  Abstract

In Gram-negative bacteria, transporters of the RND family are the most relevant in respect of resistance to antimicrobial compounds. In Pseudomonas aeruginosa, a clinically important pathogen, the RND-type pump MexAB-OprM has been recognized as one of the major multidrug efflux systems. Herein, homologues of MexAB-OprM in the plant pathogens P. syringae pv. phaseolicola 1448A, P. syringae pv. syringae B728a, and P. syringae pv. tomato DC3000 were identified and mexAB-oprM-deficient mutants were generated. Determination of minimum inhibitory concentrations revealed that mutation of MexAB-OprM dramatically reduced tolerance to a broad range of antimicrobials. Moreover, the mexAB-oprM-deficient mutants were reduced in their ability to multiply in planta. RNA dot blot hybridization revealed a growth-dependent regulation of the mexAB-oprM operon in P. syringae, being maximal expressed in early exponential phase and decreasing gradually throughout further growth.






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2008 by the American Society for Microbiology. All rights reserved.