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Appl. Environ. Microbiol. doi:10.1128/AEM.02891-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Mixed-species biofilm formation between lactic acid bacteria and rice wine yeasts

Department of Food Science and Technology, College of Bioresource Sciences, and Advanced Research Institute of the Sciences and Humanities, Nihon University

^* To whom correspondence should be addressed. Email: yamasaki-makari{at}arish.nihon-u.ac.jp.

	Abstract

Here, we found that species combinations such as Lactobacillus casei var. rhamnosus and Saccharomyces cerevisiae Kyokai-10 can form a mixed-species biofilm in co-culture. Moreover, the Kyokai-10 yeast strain can form a biofilm in monoculture in the presence of conditioned medium (CM) from L. casei IFO3831. The active substance(s) in bacterial CM is heat sensitive and has a molecular weight between 3 and 5 kDa. In biofilms from co-cultures or CM-monocultures, yeast cells had a distinct morphology, with many hill-like protrusions on the cell surface.