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Appl. Environ. Microbiol. doi:10.1128/AEM.02893-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Adherence of enterohemorrhagic Escherichia coli O157, O26 and O111 strains to bovine intestinal explants ex vivo

Divison of Cell and Molecular Biology, Imperial College London, London, Enteric Bacterial Pathogens Group, Division of Microbiology, Institute for Animal Health, Compton, Berkshire, and Centre for Paediatric Gastroenterology, Royal Free Hospital, London, United Kingdom

^* To whom correspondence should be addressed. Email: mark-p.stevens{at}bbsrc.ac.uk.

	Abstract

We use bovine intestinal organ culture to study infection with enterohemorrhagic Escherichia coli serogroups O157, O26 and O111. We show colonisation and A/E lesion formation on explants derived from the ileum, colon and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonisation.

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