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Appl. Environ. Microbiol. doi:10.1128/AEM.02923-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Temperature-dependent expression of Listeria monocytogenes internalin and internalin-like genes suggests functional diversity for these proteins among the listeriae

Department of Food Science, and Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York 14853, USA

^* To whom correspondence should be addressed. Email: kjb4{at}cornell.edu.

	Abstract

The Listeria monocytogenes genome encodes several internalins and internalin-like proteins. As L. monocytogenes is present in many environments and can infect numerous, diverse host species, environmental temperature was hypothesized as a signal that might affect internalin gene transcription. A subgenomic microarray was used to investigate temperature-dependent transcription of 24 members of the internalin gene family in L. monocytogenes 10403S. Internalin gene transcript levels for cells grown at 37°C were compared to transcript levels for cells grown at (i) 16°C, (ii) 30°C or (iii) 42°C using competitive microarray hybridization, with further confirmation by quantitative reverse transcriptase PCR (qRT-PCR) for 14 internalin genes. Based on these studies, the internalin genes can be grouped into 5 temperature-dependent categories, including (i) four ^B-dependent internalin genes (inlC2, inlD, lmo0331 and lmo0610) with highest transcript levels at 16°C and generally lowest transcript levels at 37°C; (ii) three partially PrfA-dependent internalin genes (inlA, inlB and inlC) with lowest transcript levels at 16°C and highest transcript levels at 37° and 42°C; (iii) four genes (inlG, inlJ, lmo0514 and lmo1290) with lowest transcript levels at 16°C and highest levels at 30° and/or 37°C, (iv) one gene (lmo0327) with highest transcript levels at 16°C and low transcript levels at higher temperatures, and (v) twelve internalin genes with no differences in transcript levels at the temperatures used in this study. Temperature-dependent transcription patterns suggest that the relative importance of different internalins varies by environment, which may help provide insight into the specific functions of these proteins.

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