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Appl. Environ. Microbiol. doi:10.1128/AEM.02940-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

An improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro

Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland; Cork Cancer Research Centre, University College Cork, Cork, Ireland

^* To whom correspondence should be addressed. Email: c.hill{at}ucc.ie.

	Abstract

An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections and in tumour targeting studies.

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