This Article Full Text (PDF) Other Versions of this Article: AEM.02984-06v1 73/11/3645    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Quiñones, B. Articles by Mandrell, R. E. Search for Related Content PubMed PubMed Citation Articles by Quiñones, B. Articles by Mandrell, R. E. Agricola Articles by Quiñones, B. Articles by Mandrell, R. E.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol. doi:10.1128/AEM.02984-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Detecting and Genotyping Arcobacter and Campylobacter from Retail Chicken by DNA Oligonucleotide Arrays

U.S. Department of Agriculture, Agricultural Research Service, Produce Safety and Microbiology Research Unit, Albany, California 94710

^* To whom correspondence should be addressed. Email: parker{at}pw.usda.gov.

	Abstract

To explore the use of DNA microarrays for pathogen detection in food, we have produced DNA oligonucleotide arrays to simultaneously identify the presence of Arcobacter and Campylobacter in retail chicken. Probes were selected that target housekeeping and virulence-associated genes in both Arcobacter butzleri and thermotolerant Campylobacter jejuni and Campylobacter coli. These microarrays showed a high level of probe specificity; signal intensities detected for A. butzleri, C. coli or C. jejuni probes were at least 10-fold higher than background levels. The specific identification of A. butzleri, C. coli and C. jejuni was achieved without the need of a PCR amplification step. By adapting an isolation method that employed membrane filtration and selective media, C. jejuni isolates were recovered from package liquid from whole chicken carcasses prior to enrichment. Increasing the time of enrichment resulted in the isolation of A. butzleri and increased the recovery of C. jejuni. C. jejuni isolates were further classified by using an additional subset of probes targeting the lipooligosaccharide (LOS) biosynthesis locus. Our results demonstrated that most of the C. jejuni isolates likely possess LOS classes B, C or H. Validation experiments demonstrated that this DNA microarray had a detection sensitivity threshold of approximately 10,000 C. jejuni cells. Interestingly, the use of C. jejuni-sequence specific primers to label genomic DNA improved the sensitivity of this DNA microarray for detecting C. jejuni in whole chicken carcass samples. C. jejuni was efficiently detected directly in both package liquid from whole chicken carcasses and also in enrichment broths.

This article has been cited by other articles:

• Quinones, B., Miller, W. G., Bates, A. H., Mandrell, R. E. (2009). Autoinducer-2 Production in Campylobacter jejuni Contributes to Chicken Colonization. Appl. Environ. Microbiol. 75: 281-285 [Abstract] [Full Text]  
• He, Z., Zhou, J. (2008). Empirical Evaluation of a New Method for Calculating Signal-to-Noise Ratio for Microarray Data Analysis. Appl. Environ. Microbiol. 74: 2957-2966 [Abstract] [Full Text]