Cooling Device for Use with a Sonic Oscillator

ABSTRACT

A cooling cell is described that facilitates maintenance of biological materials at low temperatures during prolonged sonic treatment. Using this cell and a Branson S-75 Sonifier, I examined temperatures and the release of protein and enzyme activity from suspensions of Saccharomyces cerevisiae. With the Sonifier at full power, it was possible to maintain cell temperature within 9 C of the cooling-bath temperature, and to disrupt 10% (w/v) suspensions of S. cerevisiae in 10 min.