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Metabolism and Products

Density-Gradient and Chromatographic Fraction of Leptospiral Lipase

Robert N. Berg, Stanley S. Green, Herbert S. Goldberg, Donald C. Blenden
Robert N. Berg
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Stanley S. Green
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Herbert S. Goldberg
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Donald C. Blenden
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ABSTRACT

Fractionation of leptospiral lipase by CsCl density gradients and G-200 Sephadex chromatography yielded five active protein peaks. Two were obtained from the density gradients and three from G-200 Sephadex columns. Esterase activity of these fractions was demonstrated by electrophoretic examination. Several protein bands were visible when disc electrophoresis was performed on the respective fractions. Lipolytic and esterolytic activities were both present, and the overlapping of these activities was discussed.

FOOTNOTES

  • ↵1 Present address: Department of Medicine, University of Illinois, Chicago, Ill.

  • ↵2 Present address: Veterinary Medicine, Walter Reed Army Institute of Research, Washington, D.C.

  • Copyright © 1969 American Society for Microbiology
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Density-Gradient and Chromatographic Fraction of Leptospiral Lipase
Robert N. Berg, Stanley S. Green, Herbert S. Goldberg, Donald C. Blenden
Applied Microbiology Mar 1969, 17 (3) 467-472; DOI:

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Density-Gradient and Chromatographic Fraction of Leptospiral Lipase
Robert N. Berg, Stanley S. Green, Herbert S. Goldberg, Donald C. Blenden
Applied Microbiology Mar 1969, 17 (3) 467-472; DOI:
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