Clinical Microbiology, Virology, and Immunology

Microculture Plaque Assay for Human and Simian Cytomegaloviruses

Shunzo Chiba, Robert L. Striker Jr., Matilda Benyesh-Melnick
DOI: 

ABSTRACT

The plaque assay for human and simian cytomegaloviruses routinely carried out in 60-mm petri dishes (macrocultures) has been adapted for use in microcultures in flat-bottom 16-mm circular wells of disposable plastic trays. Virus titrations and serum neutralization assays carried out in microcultures yielded reproducible results that were identical to those obtained in macrocultures.

FOOTNOTES

  • ↵1 On leave from Sapporo Medical College, Sapporo, Japan, as a trainee of the United States-Japan Cooperative Medical Science Program.

Back to top
Download PDF
Citation Tools
Print

Alerts
Email

Related Articles

Cited By...