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Metabolism and Products

Comparison of the Survival and Metabolic Activity of Psychrophilic and Mesophilic Yeasts Subjected to Freeze-Thaw Stress

Edward D. Meyer, Norval A. Sinclair, Bartholomew Nagy
Edward D. Meyer
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Norval A. Sinclair
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Bartholomew Nagy
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ABSTRACT

A mesophilic yeast, Candida utilis, and a psychrophilic yeast, Leucosporidium stokesii, were subjected to freeze-thaw cycling over the range 25 to -60 C. Viability after freeze-thaw stress was directly correlated with the rate of cooling and the physiological age of the cultures. Rates of glucose fermentation and oxidation could be directly correlated with viability. The optimal cooling rate for both yeast strains was 4.5 to 6.5 C/min; however, their levels of survival obtained at this optimal cooling rate varied considerably. In addition, the psychrophile was less resistant to freeze-thaw stress than was the mesophile.

  • Copyright © 1975 American Society for Microbiology
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Comparison of the Survival and Metabolic Activity of Psychrophilic and Mesophilic Yeasts Subjected to Freeze-Thaw Stress
Edward D. Meyer, Norval A. Sinclair, Bartholomew Nagy
Applied Microbiology Jun 1975, 29 (6) 739-744; DOI:

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Comparison of the Survival and Metabolic Activity of Psychrophilic and Mesophilic Yeasts Subjected to Freeze-Thaw Stress
Edward D. Meyer, Norval A. Sinclair, Bartholomew Nagy
Applied Microbiology Jun 1975, 29 (6) 739-744; DOI:
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