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Applied and Environmental Microbiology
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Applied Environmental and Public Health Microbiology

Methyl Violet: a Selective Agent for Differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and Other Gram-Negative Organisms

Lucy M. Campbell, Ivan L. Roth
Lucy M. Campbell
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Ivan L. Roth
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ABSTRACT

Three selective media for differentiation of Klebsiella pneumoniae from Enterobacter aerogenes on the basis of colonial morphology were evaluated. Using methyl violet 2B as a selective agent, strains of K. pneumoniae isolated from urine, fresh water, and fresh produce were tested against other members of Enterobacteriaceae in addition to strains of Aeromonas hydrophila and Pseudomonas aeruginosa. Comparison of colonial morphology showed K. pneumoniae produced larger, smoother colonies than other bacteria tested. These media were developed to aid in presumptive separation of K. pneumoniae from E. aerogenes in the monitoring of bacterial quality of water.

  • Copyright © 1975 American Society for Microbiology
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Methyl Violet: a Selective Agent for Differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and Other Gram-Negative Organisms
Lucy M. Campbell, Ivan L. Roth
Applied Microbiology Aug 1975, 30 (2) 258-261; DOI:

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Methyl Violet: a Selective Agent for Differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and Other Gram-Negative Organisms
Lucy M. Campbell, Ivan L. Roth
Applied Microbiology Aug 1975, 30 (2) 258-261; DOI:
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