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Applied and Environmental Microbiology
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Metabolism and Products

Derivation of High Enterotoxin B-Producing Mutants of Staphylococcus aureus from the Parent Strains

Robert A. Altenbern
Robert A. Altenbern
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ABSTRACT

Certain pH-sensitive (membrane) mutants of Staphylococcus aureus, strains 14458 and 778, produce significantly more type-B enterotoxin (SEB) than the parent type. Some carbohydrate mutants (car) from these parent strains also are superior to the parent in SEB formation. By isolating car mutants from high-SEB-producing membrane mutants, it is possible to derive a double mutant producing from six to 50 times as much SEB as the parent type. Inversion of the sequence by isolating pH-sensitive mutants from car mutants does not yield clones with strikingly higher SEB production than the parent strain. The successful isolation sequence (pH-sensitive mutant first and car mutants derived from it) is relatively simple and virtually assures detection of a truly high-SEB-producing clone. The total number of clones whose direct assay for SEB formation is necessary for detection of a high-producing mutant is on the order of 50 to 60.

  • Copyright © 1975 American Society for Microbiology
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Derivation of High Enterotoxin B-Producing Mutants of Staphylococcus aureus from the Parent Strains
Robert A. Altenbern
Applied Microbiology Aug 1975, 30 (2) 271-275; DOI:

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Derivation of High Enterotoxin B-Producing Mutants of Staphylococcus aureus from the Parent Strains
Robert A. Altenbern
Applied Microbiology Aug 1975, 30 (2) 271-275; DOI:
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