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PHYSIOLOGY AND BIOTECHNOLOGY

Microbial 7α-Hydroxylation of 3-Ketobisnorcholenol

Carl W. Despreaux, Karen R. Rittweger, Norberto J. Palleroni
Carl W. Despreaux
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Karen R. Rittweger
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Norberto J. Palleroni
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ABSTRACT

The transformation of 22-hydroxy-23,24-bisnorchol-4-en-3-one to 7α-22-dihydroxy-23,24-bisnorchol-4-en-3-one by Botryodiploida theobromae, Lasiodiplodia theobromae, and various Botryosphaeria strains is described. Factors affecting the reaction were incubation temperature, sonication of the substrate, and addition of 2,2′-dipyridyl, extra carbohydrate, and Amberlite XAD-7. The enzyme responsible for the reaction appeared to be very specific and was not characteristic of all members of the genera listed above.

FOOTNOTES

  • ↵* Corresponding author.

  • ↵† Present address: Department of Molecular Genetics, Hoffmann-La Roche, Inc.

  • ↵‡ Present address: Department of Clinical Investigation II, Hoffmann-La Roche, Inc.

  • ↵§ Present address: Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ 07331.

  • Copyright © 1986, American Society for Microbiology
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Microbial 7α-Hydroxylation of 3-Ketobisnorcholenol
Carl W. Despreaux, Karen R. Rittweger, Norberto J. Palleroni
Applied and Environmental Microbiology May 1986, 51 (5) 946-949; DOI:

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Microbial 7α-Hydroxylation of 3-Ketobisnorcholenol
Carl W. Despreaux, Karen R. Rittweger, Norberto J. Palleroni
Applied and Environmental Microbiology May 1986, 51 (5) 946-949; DOI:
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