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Research Article

High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.

M Durairaj, J L Doran, S E Jensen
M Durairaj
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J L Doran
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S E Jensen
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ABSTRACT

The pcbC gene, which encodes isopenicillin N synthase (IPNS), was subcloned from Streptomyces clavuligerus into Escherichia coli by using the pT7 series of plasmid vectors. The polymerase chain reaction was used to introduce an NdeI site at the translation initiation codon of pcbC, allowing the gene to be inserted behind an E. coli type of ribosome binding site. This construction directed high-level expression of IPNS, but the IPNS was in an inactive form in inclusion bodies. Active IPNS was recovered by solubilizing and renaturing the protein.

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High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.
M Durairaj, J L Doran, S E Jensen
Applied and Environmental Microbiology Dec 1992, 58 (12) 4038-4041; DOI:

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High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.
M Durairaj, J L Doran, S E Jensen
Applied and Environmental Microbiology Dec 1992, 58 (12) 4038-4041; DOI:
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