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Enzymology and Protein Engineering

Pressure Stabilization of Proteins from Extreme Thermophiles

Derek J. Hei, Douglas S. Clark
Derek J. Hei
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Douglas S. Clark
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ABSTRACT

We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published report of proteins from thermophiles being stabilized by pressure. Inactivation studies of partially purified hydrogenases from an extreme thermophile (Methanococcus igneus), a moderate thermophile (Methanococcus thermolithotrophicus), and a mesophile (Methanococcus maripaludis), all from shallow marine sites, show that pressure stabilization is not unique to enzymes isolated from high-pressure environments. These studies suggest that pressure stabilization of an enzyme may be related to its thermophilicity. Further experiments comparing the effects of increased pressure on the stability of α-glucosidases from the hyperthermophile Pyrococcus furiosus and Saccharomyces cerevisiae support this possibility. We have also examined pressure effects on several highly homologous glyceraldehyde-3-phosphate dehydrogenases from mesophilic and thermophilic sources and a rubredoxin from P. furiosus. The results suggest that hydrophobic interactions, which have been implicated in the stabilization of many thermophilic proteins, contribute to the pressure stabilization of enzymes from thermophiles.

FOOTNOTES

  • ↵* Corresponding author. Mailing address: Department of Chemical Engineering, University of California, 101-C Gilman Hall, Berkeley, CA 94720. Phone: (510) 642-2408. Fax: (510) 642-4778. Electronic mail address: clark@ns144.cchem.berkeley.edu.

  • ↵† Present address: Genentech, Inc., South San Francisco, CA 94080.

  • Copyright © 1994, American Society for Microbiology
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Pressure Stabilization of Proteins from Extreme Thermophiles
Derek J. Hei, Douglas S. Clark
Applied and Environmental Microbiology Mar 1994, 60 (3) 932-939; DOI:

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Pressure Stabilization of Proteins from Extreme Thermophiles
Derek J. Hei, Douglas S. Clark
Applied and Environmental Microbiology Mar 1994, 60 (3) 932-939; DOI:
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